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Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA) to detect the cytokinins in Gerbera jamesonii plantlets BABT
Sousa,Cleiton Mateus; Miranda,Ricardo Motta; Freire,Ronald Bastos.
A competitive hyper-immune yolk Immunoglobulin Y - Enzyme Linked Immune Sorbent Assay (CIgY-ELISA), was developed as an alternative method to detect zeatin and 2ip in plantlets of gerbera. The endogenous level of hormones in the plantlets in vitro of gerbera with one or six weeks after replication was determined with competitive IgY-ELISA set to detect between 1 and 100 pmoles of plant hormone for each 1.0 g tissue. The plantlets of six weeks presented sprouts and root, while the plantlets of one week presented only sprouts. The CIgY-ELISA was set with high independent variables values of sensitivity/specificity of 96/89 % for zeatin and 94/78 % for 2ip, with high values of reproducibility (up to 90 %) for both the cytokinins. Zeatin content varied from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Determination of cytokinins; CIgY-ELISA; Zeatin; 2ip; Micropropagated-Gerbera jamesonii.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400001
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Compostos fenólicos e capacidade antioxidante em frutos de tomateiros mutantes fotomorfogenéticos Ciência Rural
Dias,Tiago; Melo,Hyrandir Cabral de; Alves,Frederico Rocha Rodrigues; Carvalho,Rogério Falleiros; Carneiro,Karla da Silva; Sousa,Cleiton Mateus.
Compostos fenólicos e capacidade antioxidante são mecanismos de defesa das plantas aos danos do estresse oxidativo. Os compostos fenólicos são sintetizados pela via dos fenilpropanoides, cuja enzima chave, fenilalanina amônia liase, é influenciada pela luz e ação de fotorreceptores, como o fitocromo. O objetivo do presente trabalho é avaliar a concentração de compostos fenólicos e a capacidade antioxidante de frutos de microtomateiro selvagem, cultivar "Micro-Tom" (MT), e seus mutantes fotomorfogenéticos high pigment 1 (hp1), super-responsivo a eventos mediados por luz e aurea (au), deficiente quantitativo em fitocromos. Vinte frutos maduros de cada genótipo (MT, hp1 e au) foram utilizados para as análises, realizadas em triplicata. Para quantificação dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Áurea; Fitocromo; Hp1; Solanum lycopersicum L..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500782
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Effects of auxin and misting on the rooting of herbaceous and hardwood cuttings from the fig tree Rev. Ciênc. Agron.
Sousa,Cleiton Mateus; Busquet,Rubens Nei; Vasconcellos,Marco Antônio da Silva; Miranda,Ricardo Motta.
Propagation of the fig tree predominately uses hardwood cuttings obtained from pruning stock plants. With a view to optimising fig tree propagation, the effects of auxin and misting on the rooting of herbaceous and hardwood cuttings from the fig tree were evaluated. An experiment was set up in a 2x2x2 factorial design, with two types of cuttings (softwood and hardwood), two levels of auxin (0 and 1000 mg L-1 indolbutyric acid) and two environments (with and without misting). Thirty days after starting the experiment, rooting, root length, sprouting and losses were all evaluated. The interaction of the type of cutting and the auxin was significant for rooting, sprouting, root length and cutting loss. Misting did not affect the variables analysed. In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fig tree; Auxin; Plant propagation; Cut.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902013000200016
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Growth and production of basil under different luminosity and water replacement levels Horticultura Brasileira
Oliveira,Rívia de Souza Vaz; Salomão,Leandro Caixeta; Morgado,Hélber Souto; Sousa,Cleiton Mateus; Oliveira,Henrique Fonseca E de.
ABSTRACT Basil is used as spice and for medicinal purposes. Its growth is influenced by environmental factors, mainly soil moisture and air temperature. Thus, this study was installed to evaluate growth and production of basil under different levels of luminosity and water replacement. The experimental design was completely randomized, with six replicates, arranged in split plot scheme, with three luminosity levels in the plots (full sun, 50% and 70% shading) and four water replacement levels in the subplots (25, 50, 75 and 100% ETc). We evaluated plant height, stalk diameter, number of leaves and branches, root system volume, the largest root length, fresh and dry mass of roots and leaves. Data were submitted to variance analysis to observe significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocimum basilicum; Shading; Water deficit.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300324
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Growth of Brachiaria decumbens in Latosol contaminated with copper Ciência e Agrotecnologia
Silva,Ilmo Correia; Rocha,Cleonice; Rocha,Mariella Camargo; Sousa,Cleiton Mateus.
ABSTRACT Brachiaria decumbens presents high rusticity, rapid growth, as well as easy implantation and management. The aim of the study is to evaluate the growth of brachiaria in soil contaminated with copper. An experiment following a 3 x 4 factorial design was carried. It comprised three plant ages (30, 45 and 60 days after transplanting) and four copper concentrations (0, 20, 40 and 80 mg of Cu per kg of soil). Plant age and copper concentrations have influenced the main pseudostem length, the number of leaves, as well as the leaf area, chlorophyll (SPAD), fresh and dry shoot matter, root system volume, and fresh and dry root system matter of plants. The lowest dry shoot matter accumulation was found at the concentration 51.24 mg Kg-1. The herein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorption; Cerrado; Phytoremediation; Heavy metal..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000200168
Registros recuperados: 5
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